Daniel Greif

Bio Profile

Daniel Greif, MD

Associate Professor Term

Research Interests

Aorta; Aortic Stenosis, Subvalvular; Atherosclerosis; Cardiology; Cerebral Hemorrhage; Developmental Biology; Hypertension, Pulmonary; Pulmonary Fibrosis; Vascular Diseases

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Greif Lab


My laboratory investigates blood vessel morphogenesis, the maintenance of the adult blood vessel and diseases of the vasculature. To this end, we utilize fundamental biochemical, genetic and developmental biological approaches, including lineage and clonal analyses. We initially investigated the origins of the smooth muscle and adventitial cells of the pulmonary artery and have delineated with cellular resolution the patterns of cell differentiation, proliferation, recruitment and migration in the developing pulmonary arterial wall. Furthermore, we have initiated a genetic dissection of the molecular processes and signals that underlie blood vessel formation and have demonstrated a role of the platelet derived growth factor pathway in this process.

Publications

Greif DM, Eichmann A: Basic research: Where do new endothelial cells come from in the injured heart? Nat Rev Cardiol. 2017 Aug 3; 2017 Aug 3. PMID: 28770866

Jiao Y, Li G, Li Q, Ali R, Qin L, Li W, Qyang Y, Greif DM, Geirsson A, Humphrey JD, Tellides G: mTOR (Mechanistic Target of Rapamycin) Inhibition Decreases Mechanosignaling, Collagen Accumulation, and Stiffening of the Thoracic Aorta in Elastin-Deficient Mice. Arterioscler Thromb Vasc Biol. 2017 Jul 27; 2017 Jul 27. PMID: 28751568

Mazurek R, Dave JM, Chandran RR, Misra A, Sheikh AQ, Greif DM: Vascular Cells in Blood Vessel Wall Development and Disease. Adv Pharmacol. 2017; 2016 Oct 14. PMID: 28212800

Misra A, Sheikh AQ, Kumar A, Luo J, Zhang J, Hinton RB, Smoot L, Kaplan P, Urban Z, Qyang Y, Tellides G, Greif DM: Integrin β3 inhibition is a therapeutic strategy for supravalvular aortic stenosis. J Exp Med. 2016 Mar 7; 2016 Feb 8. PMID: 26858344

Sheikh AQ, Misra A, Rosas IO, Adams RH, Greif DM: Smooth muscle cell progenitors are primed to muscularize in pulmonary hypertension. Sci Transl Med. 2015 Oct 7. PMID: 26446956

Seidelmann SB, Lighthouse JK, Greif DM: Development and pathologies of the arterial wall. Cell Mol Life Sci. 2014 Jun; 2013 Sep 27. PMID: 24071897

Greif DM, Eichmann A: Vascular biology: Brain vessels squeezed to death. Nature. 2014 Apr 3; 2014 Mar 26. PMID: 24670635

Sheikh AQ, Lighthouse JK, Greif DM: Recapitulation of developing artery muscularization in pulmonary hypertension. Cell Rep. 2014 Mar 13; 2014 Feb 27. PMID: 24582963

Kim J, Kang Y, Kojima Y, Lighthouse JK, Hu X, Aldred MA, McLean DL, Park H, Comhair SA, Greif DM, Erzurum SC, Chun HJ: An endothelial apelin-FGF link mediated by miR-424 and miR-503 is disrupted in pulmonary arterial hypertension. Nat Med. 2013 Jan; 2012 Dec 23. PMID: 23263626

Greif DM, Kumar M, Lighthouse JK, Hum J, An A, Ding L, Red-Horse K, Espinoza FH, Olson L, Offermanns S, Krasnow MA: Radial construction of an arterial wall. Dev Cell. 2012 Sep 11. PMID: 22975322

London NR, Zhu W, Bozza FA, Smith MC, Greif DM, Sorensen LK, Chen L, Kaminoh Y, Chan AC, Passi SF, Day CW, Barnard DL, Zimmerman GA, Krasnow MA, Li DY: Targeting Robo4-dependent Slit signaling to survive the cytokine storm in sepsis and influenza. Sci Transl Med. 2010 Mar 17. PMID: 20375003

SenBanerjee S, Lin Z, Atkins GB, Greif DM, Rao RM, Kumar A, Feinberg MW, Chen Z, Simon DI, Luscinskas FW, Michel TM, Gimbrone MA Jr, García-Cardeña G, Jain MK: KLF2 Is a novel transcriptional regulator of endothelial proinflammatory activation. J Exp Med. 2004 May 17; 2004 May 10. PMID: 15136591

Greif DM, Sacks DB, Michel T: Calmodulin phosphorylation and modulation of endothelial nitric oxide synthase catalysis. Proc Natl Acad Sci U S A. 2004 Feb 3; 2004 Jan 21. PMID: 14736917

Goetz DJ, Greif DM, Shen J, Luscinskas FW: Cell-cell adhesive interactions in an in vitro flow chamber. Methods Mol Biol. 1999. PMID: 10098131

Goetz DJ, Greif DM, Ding H, Camphausen RT, Howes S, Comess KM, Snapp KR, Kansas GS, Luscinskas FW: Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin. J Cell Biol. 1997 Apr 21. PMID: 9128259